Kajian Pustaka Metode Uji Aktivitas Hipolipidemik secara In Vitro
Abstract
ABSTRACT: Hypercholesterolemia is one of the risk factors of various cardiovascular diseases. The high-risk factor needs treatment interventions such as dietary and use of natural bioactive substances, aimed at reducing lipid levels (hypolipidemic activity). The hypolipidemic activity is determined by in vitro test method. This literature study aims to determine the media, parameter, and instrument used for in vitro test of hypolipidemic activity of plant extract. The method used is the systematic literature review, which 18 articles as data sources are obtained from national and international publisher. The articles used were published in the last 10 years. The results of study revealed that cells (3T3-L1 adipocytes, Caco-2, HepG2, and HuH7), cholesterol, and enzymes (cholesterol esterase, pancreatic lipase, and HMG-CoA reductase) can be used for this test. In addition, several instruments used for in vitro test are UV-Vis spectrophotometer, HPLC, GC-FID-MS, microplate reader, luminescence spectroscopy, and GC-MS. Furthermore, the parameters of this test, namely LDL-C, HDL-C, TG, TC, % lipid accumulation, % cell inhibition, sterol concentration, increased LDL-C uptake, turbidity (OD), % LDL concentration, reduction of total cholesterol levels, % anti-cholesterol activity, % inhibition of enzymes and IC50.
Keywords: In Vitro, Media, Instruments
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ABSTRAK: Hiperkolesterolemia merupakan salah satu faktor utama terjadinya penyakit kardiovaskuler. Karena tingginya faktor resiko tersebut maka diperlukan pengobatan seperti diet dan zat bioaktif alami yang bisa menurunkan kadar lemak (hipolipidemik). Untuk melihat dan memastikan aktivitas hipolipidemik ini maka diperlukan pengujian. Salah satu metode yang digunakan yaitu metode in vitro. Kajian pustaka ini bertujuan untuk mengetahui media, parameter, dan instrumen yang digunakan. Metode yang dilakukan pada penelitian ini adalah studi literatur dengan sumber data yang digunakan sebanyak 18 artikel, baik artikel nasional maupun internasional dengan terbitan 10 tahun terakhir. Hasil kajian memperlihatkan tiga media yang dapat digunakan, yaitu sel (adiposit 3T3-L1, Caco-2, HepG2, dah HuH7), kolesterol, dan enzim (kolesterol esterase, pankreas lipase, dan HMG-KoA reduktase). Selain itu terdapat enam instrumen yang digunakan, yaitu spektrofotometer UV-Vis, HPLC, GC-FID-MS, microplate reader, spektroskopi luminesensi, dan GC-MS. Selain media dan instrumen, terdapat beberapa parameter senyawa yang digunakan yaitu, LDL-C, HDL-C, TG, TC, % akumulasi lipid, % penghambatan sel, konsentrasi sterol, peningkatan serapan LDL-C, kekeruhan (OD), % konsentrasi LDL, penurunan kadar kolesterol total, % aktivitas anti kolesterol, % penghambatan enzim dan IC50.
Kata Kunci: In Vitro, Media, Instrumen
Keywords
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DOI: http://dx.doi.org/10.29313/.v0i0.28939
