Uji In-Silico Aktivitas Melanogenesis Senyawa Turunan Betacyanin Buah Naga Merah (Hylocereus Polyrhizuz) sebagai Inhibitor Enzim Tirosinase

Dhea Nadya Mustika, Taufik Muhammad Fakih, Hilda Aprilia Wisnuwardhani

Abstract


ABSTRACT: Increased melanogenesis can lead to darkening of the skin color. Melanogenesis requires the enzyme tyrosinase. One of the phenolic compounds that have the potential as skin lightening agents is betacyanin, betalain, amaranthin, gomphrenin, bougenvillein compounds. Antioxidant activity and betacyanin, betalain, amaranthin, gomphrenin, bougenvillein compound’s plays important role as antimelanogenesis. The purpose of this study was to determine the activity of betacyanin, betalain, amaranthin, gomphrenin, bougenvillein compounds from dragon fruit in melanogenesis activity using molecular docking method. Molecular docking was carried out through the optimization stages of the compound structure using GaussView version 5.0.8 and Gaussian version 09 software, preparation of tyrosinase enzyme using BIOVIA software, validation of the docking method using MGL Tools software version 1.5.6 equipped with Autodock Tools version 4.2, docking and preparation toxicity using Toxtree software. Betacyanin compound obtained better bond energy results that betacyanin, betalain, amaranthin, gomphrenin, bougenvillein compounds with a value of -1.01 kcal/mol. Toxicity testing of betacyanin compounds, has shown potential moderate toxicity, but does not cause carcinogenicity and mutagenicity. This shows that betacyanin compound have a potential activity as a tyrosinase inhibitor.

Keywords : betacyanin, tyrosinase enzyme, melanogenesis

 ABSTRAK: Peningkatan melanogenesis dapat menyebabkan penggelapan warna kulit. Proses melanogenesis membutuhkan enzyme tyrosinase. Salah satu senyawa fenolik yang berpotensi sebagai agen pencerah kulit yaitu senyawa betacyanin, betalain, amaranthin, gomphrenin, bougenvillein. Aktivitas antioksidan dan senyawa betacyanin, betalain, amaranthin, gomphrenin, bougenvillein berperan penting dalam antimelanogenesis. Tujuan dari penelitian ini untuk mengetahui aktivitas senyawa betacyanin, betalain, amaranthin, gomphrenin, bougenvillein di dalam buah naga merah terhadap proses melanogenesis menggunakan metode molecular docking secara in silico. Molecular docking dilakukan melalui tahapan optimasi struktur senyawa menggunakan software GaussView versi 5.0.8 dan Gaussian versi 09, preparasi protein target enzyme tyrosinase menggunakan software BIOVIA, validasi metode docking menggunakan software MGL Tools versi 1.5.6 dilengkapi dengan Autodock Tools versi 4.2, docking,  preparasi toksisitas menggunakan software Toxtree. Senyawa betacyanin memperoleh hasil energi ikatan yang lebih baik dibandingkan senyawa lain (betalain, amaranthin, gomphrenin, bougenvillein) dengan nilai sebesar -1,01 kcal/mol. Pada prediksi toksisitas senyawa betacyanin memiliki potensi toksisitas yang sedang, tidak menyebabkan karsinogenitas dan mutagenitas. Hal ini menunjukkan bahwa senyawa betacyanin memiliki aktivitas  sebagai inhibitor tirosinase.

Kata Kunci : betacyanin, enzyme tyrosinase, melanogenesis


Keywords


betacyanin, enzyme tyrosinase, melanogenesis

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DOI: http://dx.doi.org/10.29313/.v0i0.29391

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