Studi in Silico Senyawa Turunan Phthalosianin terhadap Reseptor HasAp pada Bakteri Pseudomonas aeruginosa sebagai Kandidat Fotosensitizer

Eky Syahroni, Hilda Aprilia Wisnuwardhani, Taufik Muhammad Fakih

Abstract


Abstract. In developing countries especially Indonesia infectious diseases caused by bacteria are quite high so that often the occurrence of antibiotic resistance. One example of the bacterium Pseudomonas aeruginosa can lead to infections and  couse eye damage. Previous treatments such as gentamicin, cefotaxime and tobramicim has been resistant. So developed the new treatment method of photodynamic therapy (PDT) by using special drug known as photosensitizers (PS) agent so as to kill or damage target cell by using visible light at certain wavelengths. This research used the some software including ChemBioDraw Professional 16.0, ChemBio3D 16.0, Gausview 5.0.8, Gaussian 09, MGL Tools 1.5.6 which has been equipped with AutoDock Tools 4.2.3, BIOVIA Discovery Studio Visualizer 2019 and Toxtree 3.1.0. In this study used the phthalocyanin derivative compounds labeled with Nickel, Cobalt, and Copper metals against HasAp receptors owned by the Pseudomonas aeruginosa bacteria. This study aims to observe the interactions that occurred from the three compounds to the inhibition activity antibacterial  of Pseudomonas aeruginosa. The results of this study based on the testing of toxicity test compounds can still be used as antibacterial drug candidate are phthalocyanin derivative labeled with Nickel metal.

Keywords: Photosensitizers, Photodynamic, Pseudomonas aeruginosa, Receptor HasAp.

Abstrak. Di negara yang sedang berkembang khususnya Indonesia penyakit infeksi yang disebabkan oleh bakteri cukup tinggi sehingga sering terjadinya resistensi antibiotik. Salah satu contoh bakteri Pseudomonas aeruginosa yang bisa mengakibatkan infeksi serta bisa menyebabkan kerusakan mata. Pengobatan sebelumnya seperti pemberian gentamicin, sefotaksim serta tobramicim telah mengalami resistensi. Sehingga dikembangkan metode pengobatan baru berbasis photodynamic therapy (PDT) dengan menggunakan obat khusus yang dikenal dengan agen photosensitizers (PS) sehingga bisa membunuh atau merusak sel target dengan menggunakan cahaya tampak pada panjang gelombang tertentu. Penelitian ini menggunkan beberapa software diantaranya ChemBioDraw Professional 16.0, ChemBio3D 16.0, Gausview 5.0.8, Gaussian 09, MGL Tools 1.5.6 yang telah dilengkapi AutoDock Tools 4.2.3, BIOVIA Discovery Studio Visualizer 2019 dan Toxtree 3.1.0. Pada penelitian ini digunakan senyawa turunan phthalosianin yang dilabeli dengan logam Nikel, Kobalt, dan Tembaga terhadap reseptor HasAp yang dimiliki bakteri Pseudomonas aeruginosa. Penelitian ini bertujuan untuk mengamati interaksi yang terjadi  dari ketiga senyawa tersebut terhadap aktivitas penghambatan antibakteri Pseudomonas aeruginosa. Hasil dari penelitian ini berdasarkan pengujian toksisitas senyawa uji menunjukan hasil dimana senyawa tersebut masih bisa digunakan sebagai kandidat obat antibakteri adalah senyawa turunan phthalosiniani yang dilabeli logam Nikel.

Kata Kunci: Photosensitizers, Photodynamic, Pseudomonas aeruginosa, Reseptor HasAp.


Keywords


Photosensitizers, Photodynamic, Pseudomonas aeruginosa, Reseptor HasAp.

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References


Ancely, F. D. S., D. R. Q. del Almeida., L. F. Terra., S. Mauricio., B. L. Labriola. (2018). Photodynamic Therapy in Cancer Treatment-an Update Review. Jurnal of Cancer Metastatis and Treatment. Department of Biochemistry, Chemistry Institute. Universitas of Sao Paulo, Sau Paulo 05008-000, Brazil.

Chikako, S., O. Shouji., M. Terada., O. Shin-ichi., H. Sugimoto., Y. Shiro., Y. Watanabe. (2014). Inhibitor of Heme Uptake in Pseudomonas Aeruginosa by its Hemophore (HasAP) Bound to Synthetic Metal Complexes. Willey-VCH Verlag GmbH & Co. KGaA, Weinheim.

Dany, P., J. M. Lattimer., M. Prakash., A. W. Steiner. (2013). Steller Superfluids, Inspire, INT-PUB-009.

Engel, J., P. Balachandran . (2009). Role of Pseudomonas Aeruginosa Type III Effectors in Disease. Curr Opin Microbiol. 12:61-66.

Fiorillo, R., M. Zucker., D. Saywer., Lin An. (2001). The Pseudomonas Hot Food Syndrome. Engl Med 345:335-338.

Girija, C. R., Karunakar, P., Poojari, C. S., Begum, N. S., Syed, A. A. (2010). Molecular docking studies of curcumin derivative with multiple protein targets for procarcinogen activating enzyme inhibition. J Proteomics Bioinform. 3(6): 200203.

Hardjono S. (2013). Sintesis dan Uji Aktivitas Antikanker Senyawa 1-(2-Klorobenzoiloksi) urea dan 1-(4-klorobenzoiloksi) urea. Berkala Ilmiah Kimia Farmasi.

I Gusti, A.M., P. Dharmayanti., Sukrama, D. M. (2019). Karakteristik Bakteri Pseudomonas Aeruginosa dan Pola Kepekaannya Terhadap Antibiotik di Intensive Care Unit (ICU) RSUP Sanglah Pada Bulan November 2014 – Januari 2015. E-Jurnal Medika, Vol 8

Li, Qian-Shu., Xu, Xiu-Dong., Zhang, S. (2003). Predicting Energies and Geometries for Reaction Involved in Atmosphere Chemistry: A Comparison Study Between Hybrid DFT Methods. J. Chem.Phy

Lipinski, C. A., Leombardo, F., Dominy, B. W., Feeney, P. J. (2012). Experiment and computational approaches to estimate solubility and permeability in drug discovery and development setting. Advanced Drug Delivery Reviews. 23: 3-25.

Muttaqin, F. Z., R. Andriyani., S. Damayanti. (2019). Analisis In Silico Genistein dan Analognya Sebagai Inhibitor Kanker Payudara Reseptor Esterogen Alfa Positif (Era+). Bandung. ITB

Quintero, B., Miranda, M.A. (2000). Mechanism of Photosensitization Induced by Drugs. A General Survey, Ars Pharmaceutica.

Raakhee, T., Rao US. (2014). Prevalence and Resistance Pattern of Pseudomonas Strains Isolated from ICU Patients. Int. J.Curr.Microbiol. App.Sci. 3(3):527-534 12.

Roy, D., Kumar, V., Acharya, K.K., Thirumurugan, K. (2014). Probing the binding of syzygium-derived α-glucosidase inhibitors with N- and C-terminal human maltase glucoamylase by docking and molecular dynamics simulation. Applied Biochemistry Biotechnology. 172(1):102-114.

Ruswanto, Siswandono, M. Richa., N. Tita., L. Tresna. (2017). Molecular Docking of 1-Benzoyl-3methylthiourea as Anti Cancer Candidate and its Absorption, Distribution, and Toxicity Prediction. J. Pharm. Sci. & Res. 9(5): 680684.

Samuel, N. N., O. Lucy., E. Masika., M. Ng’ang’a. (2018). Antimicrobial Photodynamic Activity of Phthalocyanine Derivate. Departement of Chemecal Science and Technology, Techical University of Kenya, P.O. Box 52428-00200, Nairobi, Kenya.

Siswandono dan Soekarjdjo Bambang. (2000). Kimia Medisinal. Surabaya: Airlangga University Press.




DOI: http://dx.doi.org/10.29313/.v6i2.23035

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