Morphological Changes of Rat Liver Tissue on Long-term Soursop Leaves Extract

Abstract

The liver can experience damage induced by drug (drug induced liver injury). Soursop plants are widely used as medicines such as antioxidants, antitumors, antimicrobials and antiparasites. The aim of this study is to determine morphological changes of rat liver tissue given long-term soursop leaves extract preparation (Annona muricata). This research was an experimental in vivo study with a Randomized Complete Design against Wistar strain rats (Rattus norvegicus) which divided into four groups: positive control group (normal) and three treatment groups with dosage of 20 mg/kg body weight soursop leaf extract; 40 mg/kg body weight and 80 mg/kg body weight given over 60 days. All treatments are administered orally. After 60 days of treatment, the rats were sacrificed and the liver was taken, then the histopathologic preparations were made and viewed using a light microscope. The results of the study showed an increase in the number of swollen hepatocytes in the three treatment groups with the most significant concentration of 80 mg / kgBW, and the decrease of intact central vein without necrosis area in the three treatment groups with the most significant concentration was 40 mg / kgBW. Both illustrate the effects of injury on liver cells. The conclusions of this study indicate that the provision of long-term soursop leaves extract affects the increase in the number of hepatocytes swell and decrease the number of central vein intact.