Isolation and identification of flavonoid compound from bowl plant leaves (Polyscias scutellarium (Burm.f.) Fosb).

Siti Nur Faridatussaadah, Yani Lukmayani, Undang A Dasuki

Abstract


The research describes  Isolation and identification of flavonoid compound from bowl plant leaves (Polyscias scutellarium (Burm.f.) Fosb). The result of fitochemistry shows there is a category of alkaloid, flavonoid, saponin, triterpenoid/steroid, and polifenolat. The extraction is done by using maserasi method, it results in thick extract as many as 323,96 gram with extract bath of 32,396%. The fractsination id done using liquid-liquid extraction methode with n-heksan solvent, estate ethyl, and water. Subfracsination is done for asetate ethyl fraction with UV-Visible chromatography with elusion system. Purification process is done using preparative KLT with silent phase of silica gel GF254 and movement phase of n-heksan: acetate ethyl (6:4) up to it results isolate. Ioslate purification and characterization is tested ultra-purple- visible spectrophotometry. Characterization is then done using gestalt reagent of NaOH, AlCl3, dan AlCl3/HCl. The result of characterization concludes that isolate of bowl plant leaves (Plyscias scutellarium (Burm.f.) Fosb is flavonoid compound of auron category.

Keywords


Isolation, flavonoid, auron, bowl plant.

References


Ahmad, M.M., (2006), Anti Inflammatory Activities of Nigella sativa Linn (Kalongi, black seed), http://lailanurhayati.multiply.com/journal, diakses 22 Mei 2015.

Backer,C.A.and Bakhuizen van den Brink Jr, R.C. (1965). Flora of Java. Vol 2. Wolters Noordhoff N.V. Groningen, The Netherlands.

Dalimartha, S.(1999). Atlas Tumbuhan Obat indonesia. Jilid 1. Trubus : Jakarta.

Depkes RI, (1979), Farmakope Indonesia, ed.III,Depkes RI, Jakarta.

Depkes RI, (1995), farmakope Indonesia, ed.IV, Depkes RI, Jakarta.

Hariana, H. A. (2008). Tumbuhan Obat dan Khasiatnya, Seri 2. Cet.5 : Penebar Swadaya, Jakarta.

Harborne, J.B., (1987), Metode Fitokimia : Penuntun Cara Modern Menganalisa Tumbuhan, terjemahan Padmawita, K. dan Soediro, I., Penerbit ITB, Bandung.

Herwandi, D. (1991), Telaah Fitokimia Daun Dysoxylum Gaudichaudianum (Juss.) Miq.-Meliaceae, Skripsi Sarjana, Jurusan Farmasi, ITB.

Markham, R.K.(1988). Cara Mengidentifikasi Flavonoid.ITB : Bandung.

Ochse.J.J. and Bakhuizen van den Brink, R.C. (1931). Vegetables of The Dutch East Indies, Archipel Drukkerij, Buitenzorg.

Robinson, T. (1995). Kandungan Organik Tumbuhan Tinggi, edisi keenam, terjemahan Padmawinata, K., ITB. Bandung.

Sastrohamidjojo, H., (1991), Spektroskopi, Liberty, Yogyakarta.

Shellard, E.J., (1975),Quantitative Paper and Thin Layer Chromatography, Academic Press, New York

Townshend, A., (1995), Encyclopedia of Analitycal Science, Vol 2, Academic Press Inc, London.

Vogel, A.I., (1978),Textbook of Practical Organic Chemistry, revised by : Furniss, B.S., et al, 4th ed., Longman Group Ltd., New York.

Widjaja, E.A. (1993). Polyscias vertilicillata Stone In: Siemonsma.J.S. and Piluek.K. Plant Resources of South-East Asia Vol.8. Vegetables, Pudoc Scientific Publishers, Wageningen, the Netherlands : 226-227.




Flag Counter