Analysis of Fluoride degree In steepings tea Sample (Camellia Sinensis) Using Visible Ray Spectrophotometer method.

Abstract

Fluoride may be found in both food and beverage, one of them is tea. Tea is extremely rich in mineral, especially fluoride, since tea leaves are able to absorb soil fluoride ions and these ions are accumulated on the leaves. The fluoride in tea is useful for prevent dental caries at a certain concentration, however tea has negative impact, if it is consumed in long period of time and therefore leads to dental and bone fluoresces. The study is aimed to detect or measure fluoride found in tea steeping sample which is frequently consumed by people. The assay of fluoride ion was made by using visible ray spectrophotometer in lengthware of 587 nm using sodium 2- (para-sulfophenylazo)-1, 8-dihidroxy-3,6-naphthalene disulfonate (SPANDS)-zirconile acid reagent. This method is optimized by seeking for stability of color absorption at minute of 5 after reagent has been added. The validation of detection limit is 0,0353 ppm, quantization limit is 0,1177 ppm, and variation coefficient 2,85%. The contents of fluoride ion sample in tea steeping under measurement are varied from 26,45-0,145 ppm. The study suggests two tea steeping samples whose contents are on the range of requirements insered by WHO Fluoride & Oral Health 1994 in tea steeping should not be more than 8,6ppm.Fluoride may be found in both food and beverage, one of them is tea. Tea is extremely rich in mineral, especially fluoride, since tea leaves are able to absorb soil fluoride ions and these ions are accumulated on the leaves. The fluoride in tea is useful for prevent dental caries at a certain concentration, however tea has negative impact, if it is consumed in long period of time and therefore leads to dental and bone fluoresces. The study is aimed to detect or measure fluoride found in tea steeping sample which is frequently consumed by people. The assay of fluoride ion was made by using visible ray spectrophotometer in lengthware of 587 nm using sodium 2- (para-sulfophenylazo)-1, 8-dihidroxy-3,6-naphthalene disulfonate (SPANDS)-zirconile acid reagent. This method is optimized by seeking for stability of color absorption at minute of 5 after reagent has been added. The validation of detection limit is 0,0353 ppm, quantization limit is 0,1177 ppm, and variation coefficient 2,85%. The contents of fluoride ion sample in tea steeping under measurement are varied from 26,45-0,145 ppm. The study suggests two tea steeping samples whose contents are on the range of requirements insered by WHO Fluoride & Oral Health 1994 in tea steeping should not be more than 8,6
ppm.