Analysis of amino acid composition in capsule shells of cow gelatin estimated pork gelatin using Ultra high Performance Liquid Chromatography

Siti Nadiyaturrahmah Madani, Nety Kurniaty, Diar Herawati

Abstract


Amino acid composition analysis in cow gelatin capsule shell and supposed as pork gelatin using ultra-high performance liquid chromatography (UPLC). The stage of the study comprises the withdrawal of four samples consisted of cow gelatin supposed as pork gelatin, capsule shell with and without ‘hala’ (license) label, amino acid hydrolysis, derivation of amino acid, and amino acid profile analysis have been done. The analysis of contents of all four samples suggests that highest amino acids are glycine and proline; the highest contents of amino acids are equal the content of amino acid in earlier study (Syafiqoh, 2014) using HPLC, while the lowest content of amino acid obtained in this study is cysteinic amino acid and is found the significant difference in the amount of content as compared with earlier studies.

Keywords


UPLC, composition analysis, Amino acid, Gelatin, Capsule shell.

References


Ansel, H. C. (1989). Pengantar Bentuk Sediaan Farmasi.Edisi IV, terjemahan Ibrahim, F., Penerbit Universitas Indonesia Press, Jakarta.

Bailey,P.D.1990. An Introduction to peptide Chemistry. Wiley Interscience. New York.

Berg, J.M., John L. Tymoczko, Lubert Stryer. 0-7167-4422-8. Biochemistry fifth edition, U.S.A: W. H. Freeman and Company and Sumanas, Inc.

Fischer, G., Cao, X, Cox, N., & Francis, M. (2005). The FTIR spectra of glycine and glycylglycine zwitterions isolated in alkali halide matrices. Journal of Chemical Physicsa, 313, 39-49.

Syafiqoh, Fathmah. 2014. Analisis Gelatin Sapi dan Gelatin Babi pada Produk Cangkang Kapsul Keras Obat dan Vitamiin Menggunakan FTIR dan KCKT

Fountoulakis, M., & Lahm, H.W. 1998. Hydrolysis and amino acid composition analysis of proteins. Journal of Chromatography A, 826 (1998) 109-134

GMIA, 2012 Gelatin Handbook, USA: Gelatin Manufacturers Institute of America.

Hafidz, R.M, Yakoob, R.N, Amin L, C.M, dan Noorfaizan, A. 2011. Chemical and Functional Properties of Bovine and Porcine Skin Gelatin. International Food Research Journal 18; 813-817 (2011).

Kabelova, I., Dvorakova, M., Cizkova, H., Dostalek, P., dan Melzoch, K. 2009. Determination of free amino acids in cheeses from the Czech market. Czech J. Food Sci. Vol. 27, 2009, No. 3; 143-150.

Kealey, D and Haines, P.J, 2002, Instant Notes: Analytical Chemistry, BIOS Scientific Publishers Limited, New York

Lehninger, A. L., 1982. Principles of Biochemistry, diterjemahkan oleh Maggy Thenawidjaya, Jakarta: penerbit Erlangga.

Miller, J. C,. Miller, J. N. 2005 Statistics and Chemometrics for Analytical Chemistry Fifht Edition. Pearson Education Limited. Great Britain.

R. Ismail, A., dan Che Man, Y.B. 2012. Analytical Methods for Gelatin Differentiation from Bovine and Porcine Origins and Food Products. Journal of Food Science Vol. 71, Nr.1 2012.

Perwitasari,D.S. 2008. Hidrolisis tulang sapi menggunakan HCl untuk pembuatan gelatin. Makalah seminar nasional soebardjo brotohardjono. ISSN 1978-0427.

Poedjiadji, A. 2009. Dasar-Dasar Biokimia. Jakarta: UI-Press. Rodwell, Victor W., Kennelly, Peter J., 2000. Proteins:Determination of Primary Structure, dalam Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Rodwell, Harper’s Illustrated Biochemistry twenty-sixth edition, Amerika Serikat: Lange Medical Books/McGraw-Hill.

Rowe, R. C., Sheskey, P. J., dan Quinn, M. E. 2009. Handbook of Pharmaceutical Excipient Sixth Edition. USA: Pharmaceutical Press andAmerican Pharmacists Association 2009.

Sahilah, A.M., Mohd. Fadly, L., Norrakiah, A.S., Aminah, A., Wan Aida, W.M., Ma’aruf, A.G dan Mohd, Khan, A. 2012. Halal Market Surveillance of Soft and Hard Gel Capsules in Pharmaceutical Products Using PCR and Southern- Hybridation on the Biochip Analysis. International Food and Research Journal 19 (1): 371-375 (2012).

Singht, S., Rao, K.VR., Venugopal, K., Manikandan, R. 2002. Alteration in Dissolution Characteristics of Gelatin-Containing Formulations; A Review of the Problem. Test Methods, and Solutions. Pharmaceutical Thecnology April 2002 tersedia online pada www.pharmatech.com.

Ward,A.G. dan Courts.(1977).”The Science and Technology of Gelatin” Academic Press, New York.

Winarno, F.G. 1997. Kimia Pangan dan Gizi. Jakarta. PT. Gramedia Pustaka Utama.

Winarno, F.G. 2004. Kimia Pangan dan Gizi. Jakarta. PT. Gramedia Pustaka Utama.

Widyaninggar, A., Triwahyudi., Triyana, K, dan Ab.Rohman. 2012. Differentiation between porcine and bovine gelatin in commercial capsule shell based on amino acid profiles and principal component analysis. Indonesian J.Pharm Vol.23 No 2:96- 101 ISSN-p : 0126-1037




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