Uji In-silico Aktivitas Antikanker Prostat Senyawa Turunan Flavonoid Biji Buah Anggur Merah (Vitis vinifera) terhadap Histon Lisin Demetilase (KDM4C)
Abstract
Abstract. Cancer is one of the leading causes of death in the world. Cancer is a disease caused by abnormal cells of body tissue that grow and develop quickly and uncontrollably. One receptor that can be used as a target in prostate cancer is the enzyme histone lysine demethylase (KDM4C). Red grape seeds (Vitis vinifera) contain flavonoid derivatives namely propelargonidin, procyanidin and prodelphinidin which are known to be potential as anticancer. cancer that can be prevented by red grape seeds is prostate cancer. This study aims to determine the anticancer activity of prostate in red grape seeds (Vitis vinifera) against histone lysine demethylase (KDM4C) as a prostate cancer receptor. The test is carried out using the In-silico test. Anticancer activity of the prostate can be seen from the results of docking between compounds and receptors. The parameters observed were bond energy (∆G), constant inhibition (Ki) and the best amino acid residue bond between propelargonidin, procyanidin and prodelphinidin compounds. The results showed that Propelargonidin compound had the best binding energy value (∆G), inhibition constant (Ki) and amino acid residue with the lowest binding energy value (∆G) of -7.78 kcal / mol, the smallest value of inhibition constant (Ki) 1, 99 µM and the least amino acid residue bonds compared with procyanidin and prodelphinidin compounds.
Keywords: Prostate Anticancer, Histon Lysine Demethylase (KDM4C), Red Grape Seed (Vitis vinifera), In-silico.
Abstrak. Kanker merupakan salah satu penyebab kematian utama di dunia, Kanker adalah penyakit yang disebabkan oleh sel abnormal jaringan tubuh yang tumbuh dan berkembang dengan cepat serta tak terkendali. Salah satu reseptor yang dapat digunakan sebagai target pada kanker prostat adalah enzim histon lisin demetilase (KDM4C). Biji buah anggur merah (Vitis vinifera) mengandung senyawa turunan flavonoid yaitu propelargonidin, procyanidin dan prodelphinidin yang dikenal berpotensi sebagai antikanker. kanker yang dapat dicegah oleh biji buah anggur merah adalah kanker prostat. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker prostat pada biji buah anggur merah (Vitis vinifera) terhadap histon lisin demetilase (KDM4C) sebagai reseptor kanker prostat. Pengujian dilakukan menggunakan uji In-silico. Aktivitas antikanker prostat dilihat dari hasil docking antara senyawa dengan reseptor. Parameter yang diamati adalah energi ikatan (∆G), konstanta inhibisi (Ki) dan ikatan residu asam amino terbaik antara senyawa propelargonidin, procyanidin dan prodelphinidin. Hasil penelitian menunjukkan senyawa Propelargonidin memiliki nilai energi ikatan (∆G), konstanta inhibisi (Ki) dan residu asam amino terbaik dengan nilai energi ikatan (∆G) terkecil -7,78 kcal/mol, nilai konstanta inhibisi (Ki) terkecil 1,99 µM dan ikatan residu asam amino yang paling sedikit dibandingkan dengan senyawa procyanidin dan prodelphinidin.
Kata Kunci: Antikanker Prostat, Histon Lisin Demetilase (KDM4C), Biji Buah Anggur Merah (Vitis vinifera), In-silico
Keywords
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DOI: http://dx.doi.org/10.29313/.v6i2.24004
