Studi Literatur Aktivitas Antijamur Minyak Atsiri Tanaman Serai Cymbopogon citratus (DC) Stapf terhadap Beberapa Spesies Malassezia
Abstract
Abstract. Malassezia is a normal flora of the skin, can cause some skin problems like dandruff, tinea versicolor, seborrheic dermatitis, atopic dermatitis, etc. Antifungal commonly used to treat skin infections because Malassezia is an azole group, but can cause resistance in some people. The study of this literature study was conducted with the aim of evaluating the antifungal activity of lemongrass essential oils against various species of Malassezia. Starting with a review of several studies that tested the antifungal activity that exists in citronella essential oils against various types of Malassezia. The result is lemongrass essential oils proven to inhibit the growth of Malassezia. Then review the mechanism of action of lemongrass essential oils against Malassezia with the sorbitol & ergosterol bioassay test. The result is its antifungal mechanism by disrupting the cell membrane Malassezia, evidenced by the MIC value of essential oils which initially only 0.31-1.25 μL/mL becomes increased with the addition of ergosterol. And its antifungal activity due to citral compounds as evidenced by the TLC profile of citronella essential oils and standard citral showed patches that were parallel with RF values of 0.38. TLC plates turn purple, which indicates the presence of terpenes, citral compounds including terpenes.
Keywords: Malassezia, Antifungal, Cymbopogon citratus (DC) Stapf essential oil
Abstrak. Malassezia merupakan flora normal kulit, dapat menyebabkan beberapa masalah kulit seperti ketombe, panu, dermatitis seboroik, dermatitis atopic, dll. Antijamur yang biasa digunakan untuk mengobati infeksi kulit karena Malassezia adalah golongan azol, tetapi dapat menyebabkan resistensi pada beberapa orang. Penelitian studi literatur ini dilakukan dengan tujuan untuk mengevaulasi aktivitas antijamur dari minyak atsiri serai terhadap beragam spesies Malassezia. Dimulai dengan review beberapa penelitian yang meguji aktivitas antijamur yang ada pada minyak atsiri serai terhadap beragam jenis Malassezia. Hasilnya minyak atsiri serai terbukti dapat menghambat pertumbuhan Malassezia. Kemudian review mekanisme kerja minyak atsiri serai terhadap Malassezia dengan uji sorbitol & ergosterol bioassay. Hasilnya mekanisme antijamur nya dengan mengganggu membran sel Malassezia, dibuktikan dengan nilai MIC dari minyak atsiri yang awalnya hanya 0.31-1.25 μL/mL menjadi meningkat dengan penambahan ergosterol. Dan aktivitas antijamurnya dikarenakan senyawa citral yang dibuktikan dengan profil KLT minyak atsiri serai dan citral standar menunjukkan bercak yang sejajar dengan nilai RF 0.38. Pelat KLT menjadi berwarna ungu, yang menandakan adanya senyawa terpen, citral termasuk kedalam senyawa terpen
Kata Kunci: Malassezia, Antijamur, Minyak atsiri Cymbopogon citratus (DC) Stapf
Keywords
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DOI: http://dx.doi.org/10.29313/.v6i2.22676
