Uji Aktivitas Nefroprotektif Ekstrak Etanol Herba Seledri (Apium graveolens L.) terhadap Penurunan Kadar Serum Kreatinin Tikus Wistar Jantan yang Diinduksi Gentamisin
Abstract
Abstract. The kidneys are eliminating organs that are easily damaged by free radicals, chemicals or the use of drugs. celery Herbs (Apium graveolens L.) contain flavonoids which are nephroprotective (protect kidneys). The purpose of this study is to determine the nephroprotective activity of ethanol extract of celery herbs. Twenty-five rats were divided into five groups: normal control group, positive control group, and three extract test groups with a dose of 250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW orally. Rats induced gentamicin with a dose of 80 mg/kgBW intraperitoneally for seven days. The parameters measured were serum creatinine levels on day-0 and day-8. The results showed that the ethanol extract of celery herbs provided nephroprotective activity. Based on the Post Hoc Tukey LSD test, the ethanol extract of celery herbs at a dose of 750 mg/kgBW was the most effective dose compared to extract test at a dose of 250mg/kgBW and extract test at a dose of 500mg/kgBW seen from the most significant value with the positive control group compared to other extract test with significance value of 0.001 (p<0.05).
Keywords: Nephroprotective, Herbs of Celery (Apium graveolens L.), Gentamicin.
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Abstrak. Ginjal merupakan organ pengeliminasi yang rentan terhadap kerusakan akibat radikal bebas, zat kimia ataupun penggunaan obat-obatan. Herba seledri (Apium graveolens L.) memiliki kandungan senyawa flavonoid yang bersifat nefroprotektif (melindungi ginjal). Tujuan penelitian ini untuk mengetahui aktivitas nefroprotektif ekstrak etanol herba seledri. Dua puluh lima ekor tikus dibagi menjadi lima kelompok: kelompok kontrol normal, kelompok kontrol positif, dan tiga kelompok ekstrak uji dengan dosis masing-masing 250 mg/kgBB, 500 mg/kgBB, dan 750 mg/kgBB secara oral. Tikus diinduksi gentamisin dengan dosis 80 mg/kgBB secara intraperitoneal selama tujuh hari. Parameter yang diukur adalah kadar serum kreatinin pada hari ke-0 dan hari ke-8. Hasil penelitian menunjukkan bahwa ekstrak etanol herba seledri memberikan aktivitas nefroprotektif. Berdasarkan Uji Post Hoc Tukey LSD, ekstrak etanol herba seledri pada dosis 750 mg/kgBB merupakan dosis paling efektif dibandingkan dengan esktrak uji dosis 250mg/kgBB dan ekstrak uji dosis 500mg/kgBB dilihat dari nilai paling signifikan dengan kelompok kontrol positif dibandingkan ekstrak uji lain dengan nilai signifikansi sebesar 0,001 (p<0,05).
Kata Kunci: Nefroprotektif, Herba Seledri (Apium graveolens L.), Gentamisin.
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DOI: http://dx.doi.org/10.29313/.v0i0.18913
