Sensitivity Test Guava (Psidium guajava) Leaves Extract on Shigella dysenteriae

Dea Syahidatul Maulidiyyah, Husin U.A, Budiman Budiman


Diarrhea is the leading cause of child mortality under 5 years. The current treatment of diarrhea is antibiotics that have various side effects. In addition to antibiotics, there are various alternatives have been used for treatment. Guava leaves have been used as a traditional medicine for diarrhea. This leaf has been studied to have various benefits such as antibiotics and antidiarrhoeal. The aim of the study is to assess the antimicrobial activity of methanol extract guava leaves by measure the zone of inhibition and by determine the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on Shigella dysenteriae. This research is experimental with descriptive quantitative approach. The methods used are disk diffusion method and dilution method. The research conducted in Microbiology Laboratory of Padjadjaran Faculty of Medicine in May-June 2017 and extraction at SITH ITB Laboratory. Obtained average inhibition zone results are 15.08mm, MIC 3.125%, and MBC 6.25%. This means that this extract has a weak antibacterial effect. The active substances such as flavonoids and tannins expected as antibacterial compounds.


MIC, MBC, Psidium guajava extract, Shigella dysenteriae, Sensitivity test,


WHO. Ending Preventable Child Deaths from Pneumonia and Diarrhoea by 2025: The integrated Global Action Plan for Pneumonia and Diarrhoea (GAPPD). France: WHO; 2013.

Hegar B. Disentri. Ikatan Dokter Indonesia. 2013 (diakses 3 Februari 2017). Tersedia dari:

UNICEF. One is too many. 2016. (diakses 3 Febriari 2017) Tersedia dari :

Lulu M. WHO recommendations on the management of diarrhoea and pneumonia in HIV-infected infants and children. World Health Organization. 2010.

Gutiérrez RMP, Mitchell S, Solis RV. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2008;117(1):1–27.

M. Barbalho S, -Machado FMVF. Psidium Guajava (Guava): A Plant of Multipurpose Medicinal Applications. Med Aromat Plants. 2012;1(4):1–6.

Dhiman A, Nanda A, Ahmad S, Narasimhan B. In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava L. J Pharm bioallied Sci. 2011;3(2):226–9.

Daud E al. Pengaruh Perbedaan Metode Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Biji ( Psidium guajava L .). Pros SNaPP. 2011.

Rattanachaikunsopon P, Phumkhachorn P. Contents and antibacterial activity of flavonoids extracted from leaves of Psidium guajava. J Medinical Plants Res. 2010;4(5):393–6.

Araújoa urigena A de, Soaresb LAL, Ferreirab MRA, Netoc MA de S, Silvac GR da, Jr.d RF de A, et al. Quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone–water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajava. J Ethnopharmacol. 2014;156:88–96.

Yerhaegen JYEJ, Engbaek K, Rohner P, Piot P, Heuck CC. Prosedur Laboratorium Dasar Untuk Bakterologi Klinis. 2010; 143.

Garg N, Garg KL, Mukerji K. Laboratory Manual of Food Microbiology. New Delhi: International Publishing House; 2010;75-78.

Greenwood. Antibiotics, Susceptibility (Sensitivity) Test Antimicrobial and Chemotherapy. Mc. Graw Hill Company. USA.

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., & Yadav, A. (2013). Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. International journal of microbiology, 2013.

Cheruiyot, K. R., Olila, D., & Kateregga, J. In-vitro antibacterial activity of selected medicinal plants from Longisa region of Bomet district, Kenya. African health sciences, 2009; 9(2).

Chanu, T. R., Pai, V., Chakraborty, R., Bangar, R., Lobo, R., & Ballal, M. Screening for antidiarrheal activity of Psidium guajava: A possible alternative in the treatment against diarrhea causing enteric pathogens. Journal of Chemical and Pharmaceutical Research, 2011; 3(6): 961-967.

Lutterodt, G. D., Ismail, A., Basheer, R. H., & Baharudin, H. M.. Antimicrobial effects of Psidium guajava extract as one mechanism of its antidiarrhoeal action. The Malaysian journal of medical sciences: MJMS, 1999; 6(2):17.

Abubakar, El-Mahmood Muhammad. The use of Psidium guajava Linn. in treating wound, skin and soft tissue infections. Scientific Research and Essays, 2009; 4.6: 605-611.

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