Sensitivity Test Guava (Psidium guajava) Leaves Extract on Shigella dysenteriae

Dea Syahidatul Maulidiyyah, Husin U.A, Budiman Budiman

Abstract


Diarrhea is the leading cause of child mortality under 5 years. The current treatment of diarrhea is antibiotics that have various side effects. In addition to antibiotics, there are various alternatives have been used for treatment. Guava leaves have been used as a traditional medicine for diarrhea. This leaf has been studied to have various benefits such as antibiotics and antidiarrhoeal. The aim of the study is to assess the antimicrobial activity of methanol extract guava leaves by measure the zone of inhibition and by determine the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on Shigella dysenteriae. This research is experimental with descriptive quantitative approach. The methods used are disk diffusion method and dilution method. The research conducted in Microbiology Laboratory of Padjadjaran Faculty of Medicine in May-June 2017 and extraction at SITH ITB Laboratory. Obtained average inhibition zone results are 15.08mm, MIC 3.125%, and MBC 6.25%. This means that this extract has a weak antibacterial effect. The active substances such as flavonoids and tannins expected as antibacterial compounds.

Keywords


MIC, MBC, Psidium guajava extract, Shigella dysenteriae, Sensitivity test,

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