Pengaruh Ekstrak Ikan Gabus terhadap Proses Penyembuhan Luka pada Mencit Jantan Galur Swiss Webster

Muhammad Sidiq Karliman, Samsudin Surialaga, Hilmi Sulaiman Rathoni

Abstract


Abstract. Wounds are cellular disorders caused by exchanges with environmental energy that exceeds the body's resistance, accompanied by cell death due to ischemia or reperfusion. In several regions in Indonesia, snakehead fish is often consumed when wounds occur and in postpartum mothers. Snakehead fish meat contains albumin in the water phase and omega-3 and omega-6 fatty acids in the oil phase. In snakehead fish meat contains up to 25.1% protein and 6.224% of the protein is albumin. This study aims to determine the effect of snakehead fish meat extract on healing incision wounds in mice. This research is an experimental study using mice as experimental animals which are divided into 4 groups. Group 1 as a control, group 2 was given snakehead fish extract at a dose of 0,7 gr/kgBB, group 2 was 1,5 gr/kgBB, and group 3 was given a dose of 2.25 gr/kgBB. Data analysis was performed using the Saphiro-Wilk test for normality. Based on the measurement of wound length on day 7 of the wound epithelialization process, the p-value of the Kruskal-Wallis test, which can be seen in the first table, is 0.034. Because the p-value <0.05, it can be concluded that the dose has an effect on the assessment of wound contraction.

KeywordsExtract, Epithelialization, Snakehead Murrel, Wound Healing.

Abstrak. Luka merupakan gangguan seluler yang disebabkan pertukaran dengan energi lingkungan yang melebihi ketahanan tubuh yang disertai dengan adanya kematian sel karena iskemia ataupun reperfusi. Dibeberapa daerah di Indonesia ikan gabus seringkali dikonsumsi ketika terjadi luka dan pada ibu paska melahirkan.  Daging ikan gabus memiliki kandungan albumin pada fase airnya dan asam lemak omega-3 dan omega-6 pada fase minyaknya. Pada daging ikan gabus mengandung protein sampai 25,1% dan 6,224% dari protein tersebut adalah albumin. Penelitian ini bertujuan untuk mengetahui efek ekstrak daging ikan gabus terhadap penyembuhan luka insisi pada mencit. Penelitian ini adalah penelitian eksperimental dengan menggunakan mencit sebagai hewan coba yang dibagi menjadi 4 kelompok. Kelompok 1 sebagai kontrol, kelompok 2 diberikan ekstrak ikan gabus dengan dosis 0,7 gr/kgBB, kelompok 2 1,5 gr/kgBB, dan kelompok 3 dengan dosis 2.25 gr/kgBB. Analisis data dilakukan uji normalitas data dengan Saphiro-Wilk test. Berdasarkan pengukuran panjang luka pada hari ke 7 proses epitelisasi luka Nilai p-value uji Kruskal-Wallis, dapat dilihat pada tabel pertama, adalah 0.034. Karena  p-value < 0.05 maka dapat disimpulkan bahwa dosis berpengaruh terhadap penilaian konstraksi luka.

Kata Kunci : Ekstrak, Epitelisasi, Ikan Gabus, Penyembuhan Luka.


Keywords


Ekstrak, Epitelisasi, Ikan Gabus, Penyembuhan Luka.

References


Brunicardi FC. Schwartz’s Principles of Surgery 8th ed. 8th editio. F. Charles Brunicardi, MD F, Dana K. Andersen, MD F, Timothy R. Billiar, MD F, David L. Dunn, MD, PhD F, John G. Hunter, MD F, Raphael E. Pollock, MD, PhD F, editors. McGRAW-HILL Medical Publishing Division;

Kartika RW, Bedah B, Paru J, Luka AP. Perawatan Luka Kronis dengan Modern Dressing. 2015;42(7):546–50.

Dinas Kesehatan Republik Indonesia. Riset Kesehatan Dasar. Diabetes Mellit. 2013;87–90.

Guo S, Dipietro LA. Factors Affecting Wound Healing. 2010;(Mc 859):219–29.

Of B, Healing W. inter- leukin-1, transforming growth factor. 1999;

Williams JZ, Park JE, Barbul A. Nutrition and Wound Healing. In: Clinical Nutrition. 2005. p. 172–82.

Arnold M, Barbul A. Nutrition and Wound Healing. :42–58.

Andrie M, Sihombing D. Efektivitas Sediaan Salep yang Mengandung Ekstrak Ikan Gabus (Channa striata) pada Proses Penyembuhan Luka Akut Stadium II Terbuka pada Tikus Jantan Galur Wistar The Effectiveness of Snakehead (Channa striata) Extract- Containing Ointment on Healing Proce. Pharm Sci Res ISSN Pharm Sci Res. :2407–2354.

Asfar M, Tawali AB, Abdullah N, Mahendradatta M. Extraction Of Albumin Of Snakehead Fish ( Channa Striatus ) In Producing The Fish Protein Concentrate ( FPC ). 2014;3(4):85–8.

Shaviklo AR. Development of fish protein powder as an ingredient for food applications : a review. 2013;

Romadhoni AR, Afrianto E, Pratama RI. Extraction of snakehead fish [ophiocephalus striatus (Bloch, 1793)] into fish protein concentrate as albumin source using various solvent. J Teknol [Internet]. 2016;78(4–2):1–6. Available from: http://dx.doi.org/10.1016/j.aqpro.2016.07.001

Ariq Alauddin, Mohamad Andrie NUP, Program. Uji Efek Ekstrak Ikan Gabus (Channa Striata) Pada Luka Sayat dengan Tikus Putih Jantan Galur Wistar Yang Diberikan Secara Oral. 2016;

Kamel C, McGahan L, Mierzwinski-Urban M, Al. E. Preoperative Skin Antiseptic Preparations and Application Techniques for Preventing Surgical Site Infections: A Systematic Review of the Clinical Evidence and Guidelines [Internet]. Rapid Response Report: Summary with Critical Appraisal. 2011. 2016 p.

Cristina A, Gonzalez DO. Wound healing - A literature review *. (Figure 1):614–20.

F. Charles Brunicardi, MD F, Dana K. Andersen, MD F, Timothy R. Billiar, MD F, David L. Dunn, MD, PhD F, John G. Hunter, MD F, Raphael E. Pollock, MD, PhD F, editors. Principles of surgery. 10th ed. Vol. 10, The Laryngoscope. McGRAW-HILL Medical Publishing Division; 2014. 171–171 p.

Zakaria NKC. Pengaruh Ekstrak Ikan Gabus (Channa striata) terhadap Penyembuhan Luka Pasca Operasi Bedah Laparatomi Kucing (Felis domestica). 2015;1–39.

Listiyanto N, Andriyanto S. Ikan Gabus (Channa striata) manfaat pengembangan dan alternatif teknik budidayanya. Media Akuakultur. 2009;4(1):18–25.

Mohd Shafri MA, Abdul Manan MJ. Therapeutic potential of the haruan (Channa striatus): From food to medicinal uses. Malays J Nutr. 2012;18(1):125–36.

Baie SH, Sheikh KA. The wound healing properties of Channa striatus-cetrimide cream - tensile strength measurement. J Ethnopharmacol. 2000;71(1–2):93–100.

Rahman M, Molla M, Sarker M, Chowdhury S, Shaikh M. SF Journal of Biotechnology and Biomedical Engineering Snakehead Fish ( Channa striata ) and Its Biochemical. SF J Biotechnol Biomed Eng. 2018;1(1):1–5.

Mustafa A, Sujuti H, Permatasari N, Widodo MA. Determination of nutrient contents and amino acid composition of pasuruan Channa striata extract. IEESE Int J Sci Technol. 2013;2(4):1–11.

Mardiyanti_2016. Formulasi Serum sebagai Penyembuh Luka Bakar Berbahan Baku Utama Serbuk Konsentrat Ikan Gabus (Channa striatus) (Serum Formulation for Burn Wound Healing with The Main Raw Material is Concentrate Powder of Snakehead Fish (Channa striatus)). J Ilmu Kefarmasian Indones. 2016;14(2):181–9.

Rahayu P, Marcelline F, Sulistyaningrum E, Suhartono MT, Tjandrawinata RR. Potential effect of striatin (DLBS0333), a bioactive protein fraction isolated from Channa striata for wound treatment. Asian Pac J Trop Biomed [Internet]. 2016;6(12):1001–7. Available from: http://dx.doi.org/10.1016/j.apjtb.2016.10.008

Sahid NA, Hayati F, Rao CV, Ramely R, Sani I, Dzulkarnaen A, et al. Snakehead Consumption Enhances Wound Healing? from Tradition to Modern Clinical Practice: A Prospective Randomized Controlled Trial. Evidence-based Complement Altern Med. 2018;2018.

Article R. A simple practice guide for dose conversion between animals and human. 2016;27–31.




DOI: http://dx.doi.org/10.29313/kedokteran.v7i1.26738

Flag Counter