Studi Penambatan Molekul Senyawa Curcuma longa pada Bakteri Resisten Carbapenem Acinetobacter baumanii dengan Metode In Silico
Abstract
Abstract. The discovery of various new medicinal compounds from various research processes that further clarify the important role of computational studies as the initial basis for finding sources of medicinal raw materials both from natural and synthetic. Nosocomial infections can be caused by bacteria, viruses or other pathogens in the hospital and transmitted through equipment in the hospital. One of the bacteria that most often causes infection is Acinetobacter baumanii where these bacteria can build up resistance in the body. Method of this research is carried out in silico with the molecular docking method by looking at the docking of its compound molecules. The results showed that of the compounds tested against the receptor target, Acinetobacter Baumanii, had the ability as antibacterial, seen from the affinity bonds obtained from around -7.7 kcal / mol to -8.1 kcal / mol. Conclusion is turmeric can be used as a candidate to prevent Acinetobacter baumanii from becoming resistance.
Key words: Acinetobacter baumanii, in silico, tumeric
Abstrak. Penemuan berbagai senyawa obat baru dari berbagai proses penelitian yang semakin memperjelas peran penting studi komputasi sebagai dasar awal untuk menemukan sumber bahan baku obat baik dari alam maupun sintetis. Infeksi nosokomial dapat disebabkan oleh bakteri, virus atau patogen lain di rumah sakit dan ditularkan melalui peralatan di rumah sakit. Salah satu bakteri yang paling sering menyebabkan infeksi adalah Acinetobacter baumanii dimana bakteri tersebut dapat membangun resistensi dalam tubuh. Metode penelitian ini dilakukan secara in silico dengan metode molecular docking dengan melihat penambatan molekul senyawa yang dimilikinya. Hasil penelitian menunjukkan bahwa senyawa yang diuji terhadap target reseptor yaitu Acinetobacter baumanii memiliki kemampuan sebagai antibakteri, terlihat dari ikatan afinitas yang diperoleh dari sekitar -7,7 kkal / mol hingga -8,1 kkal / mol. Kesimpulannya, kunyit dapat digunakan sebagai kandidat untuk mencegah Acinetobacter baumanii menjadi resisten.
Kata kunci: Acinetobacter baumanii, in silico, kunyit
Keywords
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Rosenthal VD. International Nosocomial Infection Control Consortium (INICC) resources: INICC multidimensional approach and INICC surveillance online system. Am J Infect Control [Internet]. 2016;44(6):e81–90. [Diunduh tanggal 13 Februari 2020] Tersedia dari: http://dx.doi.org/10.1016/j.ajic.2016.01.005
Zinatul H, Azwar I. Pattern and antibiotics’ sensitivity of bacteria potentially causing nosocomial infection at surgical wards, RSUDZA, Banda Aceh. J Kedokt Yars. 2012;20(3):158–66.
Fournier P, Richet H, Weinstein R. The Epidemiology and control of Acinetobacter baumannii in health care facilities. Clin Infect Dis. 2006;42(5):692–9.
RSUP DI, Tirtonegoro S, Mada UG. Pola kepekaan isolat klinik. :67–83.
Saharman Y, Karuniawati A, Sedono R, Aditianingsih D, Sudarmono P, Goessens WHF. Endemic carbapenem nonsusceptible Acinetobacter baumannii calcoaceticus complex in intensive care units of the national referral hospital in Jakarta, Indonesia. Antimicrob Resist Infect Control. 2018;7(1):1–12.
Guidelines for the prevention and control of carbapenem-resistant Enterobacteriaceae, Acinetobacter baumanniiand Pseudomonas aeruginosa in health care facilities.
Maragakis LL, Perl TM. Antimicrobial resistance: Acinetobacter baumannii: Epidemiology, antimicrobial resistance, and treatment options . Clin Infect Dis. 2008;46(8):1254–63.
Indonesia Institute Of Bioinformatics. Bioinformatics Training.
Julyantika P, Dewi N, Hartiati A, Mulyani S. Pengaruh umur panen dan tingkat maserasi terhadap kandungan kurkumin dan aktivitas antioksidan ekstrak kunyit (Curcuma domestica Val.). 2016;4(3):105–15.
Teow SY, Liew K, Ali SA, Khoo ASB, Peh SC. Antibacterial action of Curcumin against Staphylococcus aureus: A Brief review. J Trop Med. 2016;2016.
Petti CA, Polage CR, Quinn TC, Ronald AR, Sande MA. Laboratory medicine in africa: a barrier to effective health care. Clin Infect Dis. 2006;42(3):377–82
Julyantika P, Dewi N, Hartiati A, Mulyani S. Pengaruh umur panen dan tingkat maserasi terhadap kandungan kurkumin dan aktivitas antioksidan ekstrak kunyit (Curcuma domestica Val.). 2016;4(3):105–15.
Kocaadam B, Åžanlier N. Curcumin an active component of turmeric ( Curcuma longa ) and its effects on health. 2017;8398.
Li S, Wang P. Chemical composition and Product quality control of turmeric ( Curcuma longa L .). 2011
Skariyachan S, Manjunath M, Bachappanavar N. Screening of potential lead molecules against prioritised targets of multi-drug-resistant-Acinetobacter baumannii–insights from molecular docking, molecular dynamic simulations and in vitro assays.JBiomolStructDyn2019;37(5):1146–69.
Protein data bank [database on internet] Protein target [diunduh 28 september 2020] tersedia dari : https://www.rcsb.org/
Garrett M, Morris, Ruth H, Arthur JO, Using Autdock for Ligan-Reseptor Docking. 2008
Pubchem [database on the internet] Ligan curcumin, demethoxycurcumin, dan bisdemethoxycurcumin [diunduh 28 september 2020] tersedia dari : https://pubchem.ncbi.nlm.nih.gov/
Pollastri MP. Overview on the rule of five. Curr Protoc Pharmacol. 2010;(Suppl. 49):1–8.
Leutz WN. Five laws for integrating medical and social services: lessons from the United States and the United Kingdom. 1999;77(1):77-110.
Supercomputing Facility for Bioinformatics and computational Biology browser [home page on the internet] lapinski’s law of five [diunduh 17 desember 2020] tersedia dari : http://www.scfbio-iitd.res.in/software/drugdesign/lipinski.jsp
Pangemanan AF, Budiarso F. Uji daya hambat ekstrak rimpang kunyit (Curcuma longa) terhadap pertumbuhan bakteri Staphylococcus aureus dan Pseudomonas sp. 2016;4(1).
Cut W. Pertumbuhan T Bacillus Uji efektivitas ekstrak kunyit sebagai antibakteri terhadap pertumbuhan bakteri. 2013.
Liang-Mei Li, Jun Li, Xiu-Ying Zhang, 2016, Antimicrobial and molecular interaction studies on derivatives of curcumin against Streptococcus pneumoniae which caused pneumonia, Electronic Journal of Biotechnology, Volume 19, Pages 8-14,
Meng XY, Zhang, Mezei M, Cui M. Molecular docking: a powerful approach for structure-based drug discovery. Current computer aided drug design. Curr Comput Aided Drug. (2011). 7(2) 146-157.
DOI: http://dx.doi.org/10.29313/kedokteran.v7i1.26735
