Efek Antimikroba Ekstrak Air Daun Mengkudu (Morinda citrifolia L.) terhadap Bakteri Shigella dysenteriae Secara In Vitro

Qyana Al Farisi, Yuke Andriane, Miranti Kania Dewi

Abstract


Abstract. Death due to infection with Shigella, especially Shigella dysenteriae, can reach more than 10%, especially in children and the elderly in conditions without effective therapy. Ciprofloxacin is the first line for the treatment of Shigella infection, however this drug has several disadvantages including high price and resistance. Noni leaf is a traditional plant that is thought to have antimicrobial effects and is expected to be an alternative antibiotic therapy for Shigella dysenteriae which is currently experiencing a lot of resistance. The purpose of this study was to determine the antimicrobial effect of the water extract of noni leaves (Morinda citrifolia L.) on Shigella dysenteriae bacteria. This research is a pure laboratory experimental study. The zone of inhibition is determined by the disc diffusion method. The research object used was Shigella dysenteriae ATCC number 13313. The test sample was a water extract of noni leaves with a concentration of 100%, positive control (ciprofloxacin) and negative control (aquadest) with 9 repetitions. The results of the antimicrobial test using the disc diffusion method showed no inhibition zone was formed in the water extract of noni leaves with a concentration of 100%. This shows that a water extract of noni leaves with a concentration of 100% did not have an antimicrobial effect against the Shigella dysenteriae bacteria. The levels of flavonoids, tannins, and alkaloids contained in the water extract of noni leaves with a concentration of 100%  in this study may not be sufficient to produce the expected effect.

Keywords: Noni Leaves, Morinda citrifolia L, Shigella dysenteriae, Shigellosis

Abstrak. Kematian akibat infeksi Shigella, terutama Shigella dysenteriae, dapat mencapai lebih dari 10% terutama pada anak-anak dan lanjut usia pada kondisi tanpa pemberian terapi yang efektif. Siprofloksasin merupakan lini pertama untuk pengobatan infeksi Shigella, akan tetapi obat ini memiliki beberapa kekurangan diantaranya harga yang mahal dan adanya resistensi.Daun mengkudu merupakan tanaman tradisional yang diduga memiliki efek antimikroba dan diharapkan dapat menjadi alternatif terapi antibiotik bagi Shigella dysenteriae yang saat ini sudah banyak mengalami resistensi. Tujuan dari penelitian ini adalah untuk mengetahui efekantimikroba ekstrak air daun mengkudu (Morinda citrifolia L.) terhadap bakteri Shigella dysenteriae. Penelitian ini bersifat eksperimentallaboratorium murni. Zona hambat ditentukan melalui metode difusi cakram. Objek penelitian yang digunakan adalah Shigella dysenteriae ATCC nomor 13313. Sampel uji berupa ekstrak air daun mengkudu konsentrasi 100%, kontrol positif (siprofloksasin) dan kontrol negatif (aquadest) dengan 9 kali pengulangan. Hasil uji antimikroba dengan metode difusi cakram menunjukkan tidak terbentuknya zona hambat padaekstrak air daun mengkudu konsentrasi 100%. Hal tersebut menunjukkanekstrak air daun mengkudu konsentrasi 100% tidak memiliki efek antimikroba terhadap bakteri Shigella dysenteriae. Kadar flavonoid, tanin, dan alkaloid yang terdapat dalam ekstrak air daun mengkudu konsentrasi 100% pada penelitian ini kemungkinan belum cukup untuk menghasilkan efek yang diharapkan.

Kata kunci: Daun Mengkudu, Morinda citrifolia L, Shigella dysenteriae, Shigellosis


Keywords


Daun Mengkudu, Morinda citrifolia L, Shigella dysenteriae, Shigellosis

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DOI: http://dx.doi.org/10.29313/kedokteran.v7i1.26652

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