Ekstrak Air Daun Sirsak mempengaruhi Mikrostruktur Kapsula Bowman secara Dose Dependent Manner pada Tikus yang Diinduksi 7,12-Dimethylbenz-[A]Anthracene (DMBA)

Faisal Akbar Athalarik, Maya Tejasari, Heni Muflihah

Abstract


Abstract. Kidney is the main excretory organ that has an important role in the human body. Damage to the kidneys can be fatal, especially damage to kidney function, so the filtration and reabsorption process does not work perfectly. 7,12-dimethylbenz- [a] anthracene (DMBA) is a toxic substance that can induce cancer and cause nephrotoxicity. Kidneys that have been exposed to DMBA will correct changes such as dilation of the bowman's capsule. It is known, the leaves of the soursop plant (Annona muricata) can increase the antioxidant Nrf-2 and release anti-inflammatory factors. The purpose of this study was to analyze how to apply soursop leaf extract to the average size of the Bowman capsule of rat kidneys. A total of 17 Wistar strain rats were used as experimental animals. This research is purely in vivo experimental research and data analysis using Anova test. Research data taken by observing rat kidney preparations that have been given assistance with a light microscope with the help of Image Raster application. The results showed the effect of doses which affected the size of the Bowman's capsule in rats. The results of statistical analysis showed a link between the deciding groups and the size of capsules. Bowman was rejected because of the value of p = 0.07 (p value> 0.05). Conclusion, give soursop leaf water extract to rats that have been induced by DMBA has no relationship statistically reviewed.

Keyword: Cancer, Annona muricata, DMBA, DMBA effect to Kidne


Abstrak.  Ginjal merupakan organ ekskresi utama yang memiliki peran penting dalam tubuh manusia. Kerusakan pada ginjal dapat berakibat fatal, terutama kerusakan pada fungsi ginjal, sehingga proses filtrasi dan reabsorpsi tidak berlangsung sempurna. 7,12-dimethylbenz-[a]anthracene (DMBA) merupakan zat toksik yang dapat menginduksi kanker dan menyebabkan nephrotoxicity. Ginjal yang telah terpapar DMBA akan mengalami perubahan seperti dilatasi kapsula bowman. Telah diketahui, daun dari tumbuhan sirsak (Annona muricata) dapat meningkatkan antioxidant Nrf- 2   dan   mengeluarkan   faktor   antiinflamasi.   Tujuan   penelitian   untuk menganalisis  pengaruh  pemberian  ekstrak  daun  sirsak  terhadap  rerata ukuran  kapsula  Bowman  ginjal  tikus.  Sebanyak  17  tikus  galur  Wistar digunakan   sebagai   hewan   coba.   Penelitian   ini   merupakan   penelitian eksperimental  murni  in  vivo  dan  analisis  data  dengan  uji  Anova.  Data penelitian diambil dengan cara melakukan observasi pada preparat ginjal tikus yang telah diberikan serangkaian perlakuan menggunakan mikroskop cahaya    dengan    bantuan    aplikasi    Image    Raster.    Hasil    penelitian menunjukkan  adanya dose dependent yang mempengaruhi ukuran kapsula Bowman ginjal  tikus. Hasil  analisis statistik  menunjukan bahwa  praduga adanya  keterkaitan  antara  kelompok  perlakuan  dengan  ukuran  kapsula Bowman  ditolak  karena  adanya  nilai  p=0.07  (nilai  p>0.05).  Simpulan, pemberian  ekstrak air daun sirsak pada tikus yang telah diinduksi DMBA tidak memiliki keterkaitan ditinjau secara statistik.

Kata Kunci: Kanker, Daun sirsak, DMBA, DMBA terhadap Ginjal

 


Keywords


Kanker, Daun sirsak, DMBA, DMBA terhadap Ginjal

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References


Candra A, Trianto HF, In’am Ilmiawan M. Gambaran Histologis Korteks Ginjal Tikus (Rattus norvegicus) Pasca Penghentian Pajanan Monosudium Glutamat per Oral. J Cerebellum. 2015;1(3):202–20.

Peh CA. Nephritis-Glomerulonephritis. In: Kidney Health Australia [Internet]. 2015. p. 1–3. Available from: http://jurnal.fk.unand.ac.id

Ola-Davies OE, Oyagbemi AA, Omobowale TO, Akande I, Ashafa A. Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: Involvement of Kim- 1/p38 MAPK/NrF2 signaling. J Basic Clin Physiol Pharmacol. 2019;30(4):1–21.

Wibowo AE, Wuyung PE, Ranasasmita R. The Influence of DMBA ( 7 , 12- dimethylbenz- [ a ] anthracene ) Regimen In The Development of Mammae Carcinogénesis on Sprague Dawley Female Rat. Indones J Cancer Chemoprevention. 2010;1(1):60–6.

Yildirim S, Ekin S, Huyut Z, Oto G, Comba A, Uyar H, et al. Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Fluoride. 2018;51(3):278–90.

Bitar R, Fakhoury R, Fahmi R, Borjac J. Histopathological Effects of the Annona muricata Aqueous Leaves Extract on the Liver and Kidneys of Albino Mice. Transl Med. 2017;07(02).

Kumar V, Abbas abdul k., Aster JC. Robbins BASIC PATHOLOGY. 9th ed. Canada; 2013.

Ozdemir H, Oto G, Ekin S, Yener Z. European Pharma Congress. JPCHS. 2015;2(4):86.

Md Roduan MR, Abd Hamid R, Mohtarrudin N. Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin. BMC Complement Altern Med. 2019;19(1):1–16.

Alnahdi H penelitian/DMBA terhadap nephrotoksisitas. pdf. S, Ayaz NO, Hussein RH, Hamza AH. Evaluation of Protective and Curative Role of Moringa Oleifera Aqueous Extract in Dimethylbenz ( a ) Anthracene ( Dmba ) Actuated - Nephrotoxic Rats. Int J Life Sci Pharma Res. 2017;7(3):L1–8.

Chen Y, Chen J wei, Zhai J hai, Wang Y, Wang S liang, Li X. Antitumor activity and toxicity relationship of annonaceous acetogenins. Food Chem Toxicol [Internet]. 2013;58:394–400. Available from: http://dx.doi.org/10.1016/j.fct.2013.05.028

M-Othman L, K-Hassan A, Elbaky AE-A, A-Mahmoud M. Antitumor activity of Annona muricata L. fruit and leaves in Ehrlich’s ascites carcinoma cell- treated mice. Int J Adv Biochem Res. 2018;2(1):21– 4.




DOI: http://dx.doi.org/10.29313/kedokteran.v0i0.21287

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